Enzyme kinetics of the human norovirus protease control virus polyprotein processing order
نویسندگان
چکیده
منابع مشابه
Polyprotein processing in Southampton virus: identification of 3C-like protease cleavage sites by in vitro mutagenesis.
A genomic clone of the small, round-structured virus Southampton virus (SV) was constructed from a set of overlapping PCR amplicons. Sequence analysis confirmed the absence of mutations and accurate ligation of the PCR products. The SV cDNA was cloned into a vector for in vitro production of RNA and subsequent translation by rabbit reticulocyte lysate. Two polypeptides corresponding to the N-te...
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15 صفحه اولCleavage map and proteolytic processing of the murine norovirus nonstructural polyprotein in infected cells.
Murine norovirus (MNV) is presently the only member of the genus Norovirus in the Caliciviridae that can be propagated in cell culture. The goal of this study was to elucidate the proteolytic processing strategy of MNV during an authentic replication cycle in cells. A proteolytic cleavage map of the ORF1 polyprotein was generated, and the virus-encoded 3C-like (3CL) proteinase (Pro) mediated cl...
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ژورنال
عنوان ژورنال: Virology
سال: 2013
ISSN: 0042-6822
DOI: 10.1016/j.virol.2013.06.013